ANALYSIS OF THE EFECTS OF AN AC ELECTROMAGNETIC FIELD EXPOSURE IN MICROCIRCULATION OF HUMANS BY USING A 2D LASER SPECKLE FLOWMETRY
Abstract
To date, there are only a few reports evaluating non-thermal alternating current electromagnetic exposure for medical therapeutic applications. Here, we analyzed the acute effects of hand and forearm exposure to a 50 Hz electromagnetic field (EMF; peak magnetic flux density Bmax 180 mT, Brms 127 mT, 15-min duration of exposure) on cutaneous microcirculation in 11 healthy human subjects (10 males and 1 female, age betwen 22-57 years). The blood flow volume values in the back of the hand were monitored and analyzed using a 2D laser speckle flowmetry. Regional blood flow volume values in sham control exposure were significantly reduced from baseline values during resting conditions. In contrast, the EMF exposure did not significantly decreased the blood flow volume from the baseline values during and after the EMF exposure period. There were significant differences between the EMF and sham exposure groups. Therefore, the EMF exposure significantly prevented the reduction of blood flow volume. Thus, the EMF could improve blood flow volume in cutaneous tissue under ischemic conditions. These findings imply that the physiological role of an EMF-enhanced blood circulation might help eliminate the metabolic waste products including endogenous pain producing substances inducing muscle hardness and pain.
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References
S. Ueno and H. Okano, Electromagnetic Fields in Biological Systems. Boca Raton, FL: CRC Press, 2011.
C. Ohkubo and H. Okano, Electromagnetic Fields in Biology and Medicine. Boca Raton, FL: CRC Press, 2015.
A. M. Begué-Simon and R. A. Drolet, “Clinical assessment of the RHUMART system based on the use of pulsed electromagnetic fields with low frequency,” International Journal of Rehabilitation Research, vol. 16, no. 4, pp. 323-327, 1993.
H. Okano, A. Fujimura, H. Ishiwatari and K. Watanuki, “The physiological influence of alternating current electromagnetic field exposure on human subjects,” in IEEE International Conference on Systems, Man, and Cybernetics (SMC), Banff, Canada, 2017, pp. 2442-2447.
I. D. Briers, “Laser Doppler and time-varying speckle: A reconciliation,” Journal of the Optical Society of America A, vol. 13, no. 2, pp. 345-350, 1996.
K. R. Forrester, C. Stewart, J. Tulip, C. Leonard and R. C. Bray, “Comparison of laser speckle and laser Doppler perfusion imaging: measurement in human skin and rabbit articular tissue,” Medical and Biological Engineering and Computing, vol. 40, no. 6, pp. 687-697, 2002.
S. Kashima, “Spectroscopic measurement of blood volume and its oxygenation in a small volume of tissue using red lasers and differential calculation between two point detections,” Optics and Laser Technology, vol. 35, no. 6, pp. 485-489. 2003.
K. Hitos, M. Cannon, S. Cannon, S. Garth and J. P. Fletcher, “Effect of leg exercises on popliteal venous blood flow during prolonged immobility of seated subjects: implications for prevention of travel-related deep vein thrombosis,” Journal of Thrombosis and Haemostasis, vol. 5, no. 9, pp. 1890-1895, 2007.
C. K. Loo, P.S. Sachdev, W. Haindl, W. Wen, P. B. Mitchell, V. M. Croker and G.S. Malhi, “High (15 Hz) and low (1 Hz) frequency transcranial magnetic stimulation have different acute effects on regional cerebral blood flow in depressed patients,” Psychological Medicine, vol. 33, no. 6, pp. 997-1006, 2003.
T. T. Cao, R. H. Thomson, N. W. Bailey, N. C. Rogasch, R. A. Segrave, J. J. Maller, Z. J. Daskalakis and P. B. Fitzgerald, “A near infra-red study of blood oxygenation changes resulting from high and low frequency repetitive transcranial magnetic stimulation,” Brain Stimulation, vol. 6, no. 6, pp. 922-924, 2013.
Y. Li, J. W. Hand, T. Wills and J. V. Hajnal, “Numerically-simulated induced electric field and current density within a human model located close to a z-gradient coil,” Journal of Magnetic Resonance Imaging, vol. 26, no. 5, pp. 1286-1295, 2007.
S. Gabriel, R. W. Lau and C. Gabriel, “The dielectric properties of biological tissues: III. Parametric models for the dielectric spectrum of tissues,” Physics in Medicine and Biology, vol. 41, no. 11, pp. 2271-2293, 1996.
M. Lu and S. Ueno, “Comparison of the induced fields using different coil configurations during deep transcranial magnetic stimulation,” PLoS One, vol. 12, no. 6, pp. 1-12, 2017.
T. Wagner, U. Eden, F. Fregni, A. Valero-Cabre, C. Ramos-Estebanez, V. Pronio-Stelluto, A. Grodzinsky, M. Zahn and A. Pascual-Leone, “Transcranial magnetic stimulation and brain atrophy: a computer-based human brain model study,” Experimental Brain Research, vol. 186, no. 4, pp. 539-550, 2008.
World Health Organization (WHO). (1987). Environmental Health Criteria 69: Magnetic Fields [Online]. Available FTP: http://www.inchem.org/documents/ehc/ehc/ehc69.htm
J. A. Robertson, A. W. Thomas, Y. Bureau and F. S. Prato, “The influence of extremely low frequency magnetic fields on cytoprotection and repair,” Bioelectromagnetics, vol. 28, no. 1, pp. 16-30. 2007.
S. Ravera, B. Bianco, C. Cugnoli, I. Panfoli, D. Calzia, A. Morelli and I. M. Pepe, “Sinusoidal ELF magnetic fields affect acetylcholinesterase activity in cerebellum synaptosomal membranes,” Bioelectromagnetics, vol. 31, no. 4, pp. 270-276, 2010.
A. Patruno, P. Amerio, M. Pesce, G. Vianale, S. Di Luzio, A. Tulli, S. Franceschelli, A. Grilli, R. Muraro and M. Reale, “Extremely low frequency electromagnetic fields modulate expression of inducible nitric oxide synthase, endothelial nitric oxide synthase and cyclooxygenase-2 in the human keratinocyte cell line HaCat: potential therapeutic effects in wound healing,” British Journal of Dermatology, vol. 162, no. 2, pp. 258-266, 2010.
N. Cichoń, P. Czarny, M. Bijak, E. Miller, T. Śliwiński, . Szemraj and J. Saluk-Bijak, “Benign effect of extremely low-frequency electromagnetic field on brain plasticity assessed by nitric oxide metabolism during poststroke rehabilitation,” Oxidative Medicine and Cellular Longevity, vol. 2017, pp. 1-9, 2017.
N. Toda, K. Ayajiki and T. Okamura, “Cerebral blood flow regulation by nitric oxide: recent advances,” Pharmacological Reviews, vol. 61, no. 1, pp. 62-97, 2009.
G. E. Plante, “Vascular response to stress in health and disease,” Metabolism, vol. 51, no. 6PB, pp. 25-30, 2002.
